Human Sandwich TIMP-1 ELISA Kit

70-EK1138

Product Name

Human TIMP-1 ELISA Kit

Customs Name

Human TIMP-1 ELISA Kit

Application

ELISA

Reactivity

Human

Assay Type

Sandwich ELISA

Suitable Sample Type

serum, plasma, cell culture supernates

Format

96-well strip plate

Storage

4? (unopened) standard stored at -20?, others stored at 4? (opened)

Shipping Condition

4?

Sample Volume

10 µl (prediluted)

Sensitivity

3.94 pg/ml

Standard Curve Range

31.25 - 2000 pg/ml

Spike Recovery Range

-

Mean Spike Recovery

-

CV of Intra plate

4.9 % - 5.4 %

CV of Inter plate

6.4 % - 7.3 %

Components

96-well polystyrene microplate coated with a monoclonal antibody against TIMP-1

Human TIMP-1 Standard, lyophilized

TIMP-1 Detect Antibody

Streptavidin-HRP

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human TIMP-1. The Human TIMP-1 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Tissue inhibitor of matrix metalloprotease-1 (TIMP-1) is a glycoprotein that is expressed from the several tissues of organisms. This protein is a member of the TIMP family. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function.

Increased expression of TIMP-1 has been found to be associated with worse prognosis of various tumors, such as laryngeal carcinoma or melanoma.