Mouse M-CSF ELISA Kit Plate

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Product Detail

Standard - EK2144.jpg

BrandMultiSciences
CatNum70-EK2144
ProductNameMouse M-CSF ELISA Kit
CustomsNameMouse M-CSF ELISA Kit
ApplicationELISA
ReactivityMouse
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4? (unopened) standard stored at -20?, others stored at 4? (opened)
Shipping Condition4?
Sample Volume20 µl
Sensitivity1.89 pg/ml
Standard Curve Range31.25 - 2000 pg/ml
Spike Recovery Range90 % - 108 %
Mean Spike Recovery1.01
CV of Intra plate4.6 % - 7.0%
CV of Inter plate3.2 % - 4.7 %
Components96-well polystyrene microplate coated with a monoclonal antibody against IFN-?
Mouse IFN-? Standard, lyophilized
IFN-? Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse M-CSF. The Mouse M-CSF ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Macrophage colony-stimulating factor (M-CSF), also known as colony stimulating factor 1 (CSF1), is a secreted cytokine released by osteoblasts. It is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. Locally produced M-CSF in the vessel wall contributes to the development and progression of atherosclerosis. M-CSF has been described to play a role in renal pathology including Acute kidney injury (AKI) and Chronic Renal Failure (CRF). The chronic activation of monocytes can lead to multiple metabolic, hematologic and immunologic abnormalities in patients with CRF. In the context of AKI, M-CSF has been implicated in promoting repair following AKI, but also been described in an opposing role, driving proliferation of a pro-inflammatory macrophage phenotype.
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