Rat IgM Antibody ELISA Kit

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Product Detail

Standard - EK376.jpg


BrandMultiSciences
CatNum70-EK376
ProductNameRat IgM ELISA Kit
CustomsNameRat IgM ELISA Kit
ApplicationELISA
ReactivityRat
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4? (unopened) standard stored at -20?, others stored at 4? (opened)
Shipping Condition4?
Sample Volume20 µl (prediluted)
Sensitivity0.17 ng/ml
Standard Curve Range0.63 - 40 ng/ml
Spike Recovery Range-
Mean Spike Recovery-
CV of Intra plate4.8 % - 7.7 %
CV of Inter plate6.1 % - 7.1 %
Components96-well polystyrene microplate coated with a monoclonal antibody against IgM
Rat IgM Standard, lyophilized
IgM Detect Antibody
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat IgM. The Rat IgM ELISA is for research use only. Not for use in therapeutic procedures.
Immunoglobulin M (IgM) is a basic antibody that is produced by B cells. It is the first antibody to appear in response to initial exposure to an antigen. The spleen, where plasmablasts responsible for antibody production reside, is the major site of specific IgM production. IgM in normal serum is often found to bind to specific antigens, even in the absence of prior immunization. For this reason IgM has sometimes been called a "natural antibody". Demonstrating IgM antibodies in a patient's serum indicates recent infection, or in a neonate's serum indicates intrauterine infection.

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