Rat TGF-Beta1 Assay ELISA Kit

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Standard - EK381.jpg

Brand	MultiSciences
CatNum	70-EK381
ProductName	Rat TGF-beta1 ELISA Kit
CustomsName	Rat TGF-beta1 ELISA Kit
Application	ELISA
Reactivity	Rat
Assay Type	Sandwich ELISA
Suitable Sample Type	serum, plasma, cell culture supernates
Format	96-well strip plate
Storage	4? (unopened) standard stored at -20?, others stored at 4? (opened)
Shipping Condition	4?
Sample Volume	50 µl (prediluted)
Sensitivity	3.36 pg/ml
Standard Curve Range	31.25 - 2000 pg/ml
Spike Recovery Range	83 % - 117 %
Mean Spike Recovery	0.99
CV of Intra plate	2.0 % - 5.3 %
CV of Inter plate	4.1 % - 6.0 %
Components	96-well polystyrene microplate coated with a monoclonal antibody against TGF-ß1 Rat TGF-ß1 Standard, lyophilized TGF-ß1 Detect Antibody Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) HCl NaOH Plate Covers
Describtion	This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat TGF-ß1. The Rat TGF-ß1 ELISA is for research use only. Not for diagnostic or therapeutic procedures. Transforming growth factor beta 1 (TGF-ß1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines that performs many cellular functions, including the control of cell growth,cell proliferation, cell differentiation and apoptosis. TGF-ßs are a multifunctional set peptides that controlsproliferation, differentiation, and other functions in many cell types. TGF-ßs act synergistically with TGFA in inducingtransformation. It also acts as a negative autocrine growth factor. Dysregulation of TGF-ß activation and signaling may result in apoptosis. Many cells synthesize TGF-ß and almost all of them have specific receptors for this peptide. TGF-ß1, TGF-ß2 , and TGF-ß3 all function through the same receptor signaling systems. TGF-ß1 plays an important role in controlling the immune system, and shows different activities on different types of cell, or cells at different developmental stages. Most immune cells (orleukocytes) secrete TGF-ß1. TGF-ß1 is related to cancer, autoimmune diseases, liver diseases, kidney diseases, diabetes, cardiovascular diseases, asthma, chronic obstructive pulmonary disorder (COPD), cystic fibrosis (CF) and so on.