Rat CXCL1/KC Protein A ELISA Kit

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Product Detail

Standard - EK396.jpg

BrandMultiSciences
CatNum70-EK396
ProductNameRat CXCL1/KC ELISA Kit
CustomsNameRat CXCL1/KC ELISA Kit
ApplicationELISA
ReactivityRat
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4? (unopened) standard stored at -20?, others stored at 4? (opened)
Shipping Condition4?
Sample Volume20 µl
Sensitivity0.26 pg/ml
Standard Curve Range15.63 - 1000 pg/ml
Spike Recovery Range92 % - 109 %
Mean Spike Recovery1
CV of Intra plate3.3 % - 5.4 %
CV of Inter plate3.5 % - 5.7 %
Components96-well polystyrene microplate coated with a monoclonal antibody against CXCL1
Rat CXCL1 Standard, lyophilized
CXCL1 Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat CXCL1. The Rat CXCL1 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Chemokine (C-X-C motif) ligand 1 (CXCL1), also known as KC or N51, is a small cytokine belonging to the CXC chemokine family. It was originally identified in fibroblasts as a PDGF-induced immediate early gene that encodes a secretory protein of approximately 8 kDa.
CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing, and tumorigenesis. CXCL1 is induced by inflammatory cytokines such as IL-1 and TNF, and IL-17A enhances expression of CXCL1 by prolonging the half-life of its constitutively unstable mRNA. An initial study in mice showed evidence that CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function.
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